Covers

A full list of publications can be found here.

Recent publications

• Pagnier A, Balci B, Shepard EM, Yang H, Drena A, Holliday GL, Hoffman BM, Broderick WE, Broderick JB. (2024) Role of ammonia-lyases in the synthesis of the dithiomethylamine ligand during [FeFe]-hydrogenase maturation.Journal of Biological Chemistry: v. 300 i. 10 p. 107760 (1-13). 
• Walls, W. G., Vagstad, A. L., Delridge, T., Piel, J., Broderick, W. E., Broderick, J. B. (2024) Direct Detection of the α-Carbon Radical Intermediate Formed by OspD: Mechanistic Insights into Radical S-Adenosyl-l-methionine Peptide Epimerization.Journal of the American Chemical Society: v. 146 i. 8 p. 5550-5559
• Yang, H., Ho, M. B., Lundahl, M. N., Mosquera, M. A., Broderick, W. E., Broderick, J. B., Hoffman, B. M. ENDOR Spectroscopy Reveals the "Free" 5'-Deoxyadenosyl Radical in a Radical SAM Enzyme Active Site Actually is Chaperoned by Close Interaction with the Methionine-Bound [4Fe-4S]²⁺ Cluster. Journal of the American Chemical Society:  v. 146 i. 6 p. 3710-3720
• Fausset, H., Spietz, R. L., Cox, S., Cooper, G., Spurzem, S., Tokmina-Lukaszewska, M., DuBois, J., Broderick, J. B., Shepard, E. M., Boyd, E. S., Bothner, B. (2024) A shift between mineral and nonmineral sources of iron and sulfur causes proteome-wide changes in Methanosarcina barkeri.Microbiology Spectrum: v. 12 i. 2 p. e0041823
• Lundahl, M. N., Yang, H., Broderick, W. E., Hoffman, B. M., Broderick, J. B. (2023) Pyruvate formate-lyase activating enzyme: The catalytically active 5'-deoxyadenosyl radical caught in the act of H-atom abstraction. Proceedings of  the National Academy of Science of the united States of America: v. 120 i. 47 p. e2314696120 
• Balci, B., O'Neill, R. D. , Shepard, E. M., Pagnier, A., Marlott, A., Mock, M. T. , Broderick W. E., Broderick J. B. (2023) Semisynthetic maturation of [FeFe]-ydrogenase using [Fe₂(μ-SH)₂(CN)₂(CO)₄]^2-: key roles for HydF and GTP.Chemical Communications (Camb): v. 18 i. 59(58) p. 8929-8932. 
• Jodts, R. J., Wittkop, M., Ho, M. B., Broderick, W. E., Broderick, J. B., Hoffman, B. M., Mosquera, M. A. (2023) Computational Description of Alkylated Iron-Sulfur Organometallic Clusters.Journal of the American Chemical Society: v. 145 i. 25 p. 13879-13887
• Moody, J. D., Hill, S., Lundahl, M. N., Saxton, A. J., Galambas, A., Broderick, W. E., Lawrence, C. M., Broderick, J. B. (2023) Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage.Journal of Biological Chemistry: v. 299 i. 6 p. 104791
• Hoffman, B.M., Broderick, W.E., Broderick, J. B. (2023) Mechanism of Radical Initiation in the Radical SAM Enzyme Superfamily.Annual Review of Biochemistry: v. 92 p. 333-349
• Broderick, J. B., Broderick, W. E., Hoffman, B. M. (2023) Radical SAM enzymes: Nature's choice for radical reactions. FEBS Letters: v. 597 i. 1 p. 92-101
• Lundahl, M. N., Sarksian, R., Yang, H., Jodts, R. J., Pagnier, A., Smith, D. F., Mosquera, M. A., van der Donk, Wilfred A., Hoffman, B. M., Broderick, J. (2022) Mechanism of RadicalS-Adenosyl-L-methionine Adenosylation:Radical Intermediates and the Catalytic Competence of the 5 '-Deoxyadenosyl Radical.Journal of the American Chemical Society: v. 144 i. 11 p. 5087-5098
• Pagnier, A., Balci, B., Shepard, E. M., Broderick, W. E., Broderick, J. (2022) [FeFe]-Hydrogenase In Vitro Maturation. Angewandte Chemie, International Edition: v. 61 i. 49 p. e202212074
• Steward, K. F., Payne, D., Kincannon, W., Johnson, C., Lensing, M., Fausset, H., Nemeth, B., Shepard, E. M., Broderick, W. E., Broderick, J.,Bothner, B. (2022) Proteomic Analysis of Methanococcus voltae Grown in the Presence of Mineral and Nonmineral Sources of Iron and Sulfur. Microbiology Spectrum: v. 10 i. 4 p. e01893-22
• Pagnier, A., Balci, B., Shepard, E. M., Yang, H., Warui, D. M., Impano, S., Booker, S. J., Hoffman, B. M., Broderick, W. E., Broderick, J. (2022) “[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis. Angewandte Chemie, International Edition: v. 61 i. 22 p. e202203413
• Walls, W., Moody, J., McDaniel, E., Villanueva, M., Shepard, E. M., Broderick, W., Broderick, J. (2022) The B12-independent glycerol dehydratase activating enzyme from Clostridium butyricum cleaves SAM to produce 5'-deoxyadenosine and not 5'-deoxy-5'-(methylthio)adenosine. Journal of Inorganic Biochemistry: v. 227 p. 10
• Payne, D., Shepard, E. M., Spietz, R., Steward, K., Brumfield, S., Young, M., Bothner, B., Broderick, W., Broderick, J., Boyd, E. (2021) Examining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells. Journal of Bacteriology: v. 203 i. 19
• Broderick, J. B., Lundahl, M., Nemeth, B., Broderick, W. (2021) Radical S-Adenosyl-L-methionine Enzymes. Comprehensive Coordination Chemistry III. Elsevier: p. 124-133
• Shepard, E. M., Impano, S., Duffus, B., Pagnier, A., Duschene, K., Jeremiah, B., Byer, A., Galambas, A., McDaniel, E.,Broderick, J. (2021) HydG, the "dangler" iron, and catalytic production of free CO and CN-: implications for [FeFe]-hydrogenase maturation. Dalton Transactions: v. 50 i. 30 p. 10405-10422
• Impano, S., Yang, H., Shepard, E. M., Swimley, R., Pagnier, A., Broderick, W., Hoffman, B. M., Broderick, J. (2021) S-Adenosyl-l-ethionine is a Catalytically Competent Analog of S-Adenosyl-l-methionine (SAM) in the Radical SAM Enzyme HydG. Angewandte Chemie International Edition: v. 60 i. 9 p. 4666-4672